Identification of differentially expressed genes in Dreissena polymorpha exposed to contaminants.
Identifieur interne : 001733 ( France/Analysis ); précédent : 001732; suivant : 001734Identification of differentially expressed genes in Dreissena polymorpha exposed to contaminants.
Auteurs : F. Bultelle [France] ; M. Panchout ; F. Leboulenger ; J M DangerSource :
- Marine environmental research [ 0141-1136 ]
English descriptors
- KwdEn :
- Animals, Base Sequence, Bivalvia (genetics), Bivalvia (physiology), DNA Primers, Environmental Exposure, Forecasting, Gene Expression Regulation, Gene Library, Genetic Markers, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction (methods), RNA, Messenger (analysis), RNA, Messenger (biosynthesis), Water Pollutants, Chemical (adverse effects).
- MESH :
- chemical , adverse effects : Water Pollutants, Chemical.
- chemical , analysis : RNA, Messenger.
- chemical , biosynthesis : RNA, Messenger.
- chemical : DNA Primers, Genetic Markers.
- genetics : Bivalvia.
- methods : Polymerase Chain Reaction.
- physiology : Bivalvia.
- Animals, Base Sequence, Environmental Exposure, Forecasting, Gene Expression Regulation, Gene Library, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis.
Abstract
Development of transcriptome analysis methods such as differential display PCR and construction of subtractive libraries now makes it possible to profile gene expression in response to xenobiotic exposure. As an example of application of these methods, zebra mussels (Dreissena polymorpha) were treated with various contaminants such as Aroclor 1254, 3-methylcholanthrene, chrysene and atrazine. A total of 242 mRNAs were identified as differentially expressed. Analysis of these mRNAs should provide valuable information regarding detoxification mechanisms in this bivalve species. In addition, the use of cDNA array technology applied to these gene products may constitute a multi-marker approach to monitor the effect of contamination on this aquatic species.
PubMed: 12408591
Affiliations:
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pubmed:12408591Le document en format XML
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Panchout</name></author><author><name sortKey="Leboulenger, F" sort="Leboulenger, F" uniqKey="Leboulenger F" first="F" last="Leboulenger">F. Leboulenger</name></author><author><name sortKey="Danger, J M" sort="Danger, J M" uniqKey="Danger J" first="J M" last="Danger">J M Danger</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2002 Sep-Dec</MedlineDate></PubDate></date><idno type="RBID">pubmed:12408591</idno><idno type="pmid">12408591</idno><idno type="wicri:Area/PubMed/Corpus">000368</idno><idno type="wicri:Area/PubMed/Curation">000368</idno><idno type="wicri:Area/PubMed/Checkpoint">000368</idno><idno type="wicri:Area/Ncbi/Merge">000057</idno><idno type="wicri:Area/Ncbi/Curation">000057</idno><idno type="wicri:Area/Ncbi/Checkpoint">000057</idno><idno type="wicri:doubleKey">0141-1136::Bultelle F:identification:of:differentially</idno><idno type="wicri:Area/Main/Merge">003413</idno><idno type="wicri:Area/Main/Curation">003326</idno><idno type="wicri:Area/Main/Exploration">003326</idno><idno type="wicri:Area/France/Extraction">001733</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of differentially expressed genes in Dreissena polymorpha exposed to contaminants.</title><author><name sortKey="Bultelle, F" sort="Bultelle, F" uniqKey="Bultelle F" first="F" last="Bultelle">F. Bultelle</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Ecotoxicology, UPRES-EA 3222, IFRMP23, University of Le Havre, France. florence.bultelle@univ-lehavre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Ecotoxicology, UPRES-EA 3222, IFRMP23, University of Le Havre</wicri:regionArea><wicri:noRegion>University of Le Havre</wicri:noRegion><wicri:noRegion>University of Le Havre</wicri:noRegion></affiliation></author><author><name sortKey="Panchout, M" sort="Panchout, M" uniqKey="Panchout M" first="M" last="Panchout">M. Panchout</name></author><author><name sortKey="Leboulenger, F" sort="Leboulenger, F" uniqKey="Leboulenger F" first="F" last="Leboulenger">F. Leboulenger</name></author><author><name sortKey="Danger, J M" sort="Danger, J M" uniqKey="Danger J" first="J M" last="Danger">J M Danger</name></author></analytic><series><title level="j">Marine environmental research</title><idno type="ISSN">0141-1136</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Bivalvia (genetics)</term><term>Bivalvia (physiology)</term><term>DNA Primers</term><term>Environmental Exposure</term><term>Forecasting</term><term>Gene Expression Regulation</term><term>Gene Library</term><term>Genetic Markers</term><term>Molecular Sequence Data</term><term>Oligonucleotide Array Sequence Analysis</term><term>Polymerase Chain Reaction (methods)</term><term>RNA, Messenger (analysis)</term><term>RNA, Messenger (biosynthesis)</term><term>Water Pollutants, Chemical (adverse effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="adverse effects" xml:lang="en"><term>Water Pollutants, Chemical</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term><term>Genetic Markers</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bivalvia</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Bivalvia</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Environmental Exposure</term><term>Forecasting</term><term>Gene Expression Regulation</term><term>Gene Library</term><term>Molecular Sequence Data</term><term>Oligonucleotide Array Sequence Analysis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Development of transcriptome analysis methods such as differential display PCR and construction of subtractive libraries now makes it possible to profile gene expression in response to xenobiotic exposure. As an example of application of these methods, zebra mussels (Dreissena polymorpha) were treated with various contaminants such as Aroclor 1254, 3-methylcholanthrene, chrysene and atrazine. A total of 242 mRNAs were identified as differentially expressed. Analysis of these mRNAs should provide valuable information regarding detoxification mechanisms in this bivalve species. In addition, the use of cDNA array technology applied to these gene products may constitute a multi-marker approach to monitor the effect of contamination on this aquatic species.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><noCountry><name sortKey="Danger, J M" sort="Danger, J M" uniqKey="Danger J" first="J M" last="Danger">J M Danger</name><name sortKey="Leboulenger, F" sort="Leboulenger, F" uniqKey="Leboulenger F" first="F" last="Leboulenger">F. Leboulenger</name><name sortKey="Panchout, M" sort="Panchout, M" uniqKey="Panchout M" first="M" last="Panchout">M. Panchout</name></noCountry><country name="France"><noRegion><name sortKey="Bultelle, F" sort="Bultelle, F" uniqKey="Bultelle F" first="F" last="Bultelle">F. Bultelle</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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